Part:BBa_K1335000:Design

ATP-ase from phage Gp16

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGACAAGAGTTTATTTTATAATCCACAGAAAATGTTATCATACGATCGCATACTGAACTTTGTTATCGGTGCTCGTGGTATCGGTAAATCATATGCAATGAAGGTGTACCCTATTAATCGCTTTATTAAGTACGGAGAACAATTCATATATGTTCGACGATACAAACCGGAGCTTGCGAAGGTCTCCAACTATTTTAATGATGTAGCTCAAGAGTTCCCTGACCATGAGTTGGTTGTGAAGGGTAGAAGGTTCTACATTGATGGTAAGCTTGCAGGGTGGGCTATTCCTCTGAGTGTGTGGCAGAGTGAAAAATCTAATGCATATCCTAACGTAAGCACAATAGTATTTGATGAGTTTATCAGGGAGAAAGACAATAGCAACTATATTCCTAATGAGGTTTCAGCTTTACTAAACCTTATGGACACCGTATTTCGTAACCGTGAGCGTGTCAGATGCATTTGTTTAAGTAATGCTGTATCCGTTGTTAACCCTTATTTTCTGTTCTTCAACCTTGTCCCTGATGTCAACAAACGCTTCAATGTATATGACGATGCTTTAATTGAAATACCTGATAGTCTTGACTTCTCATCTGAAAGGCGTAAAACAAGGTTTGGGCGGCTAATTGATGGAACCGAGTACGGTGAGATGAGTTTAGATAACCAGTTTATCGGAGATAGTCAGGTGTTTATAGAAAAGCGCAGTAAGGATAGTAAGTTTGTATTCTCCATCGTCTATAATGGATTCACTCTTGGTGTGTGGGTTGATGTTAATCAAGGTCTTATGTACATTGATACAGCACATGACCCGTCAACTAAGAATGTATACACATTGACAACAGATGATCTTAATGAAAACATGATGTTGATAACCAACTATAAGAATAATTATCATTTACGTAAGTTAGCTAGTGCGTTCATGAATGGTTATCTGAGGTTTGACAATCAGGTTATCAGAAATATCGCATATGAGTTGTTTCGTAAGATGCGTATACAGTAAGTTTCTTCCTGCAGCGGCCGCTACTAGTA This gene is in pUC57 plasmid

Source

Synthesized by GenScript company with point mutation to remove EcoRI site